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                      關鍵詞搜索: 3D細胞培養相關產品,多肽毒素,原代細胞,細胞因子,抗體,試劑盒,各類細菌、病毒熒光定量PCR試劑盒,轉基因檢測儀器和耗材等。

                      你的位置:首頁 > 產品展示 > > 細胞生物學檢測試劑盒 >Biocolor 硫酸氨基聚糖檢測試劑盒(Blyscan™ Glycosaminoglycan As

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                      Biocolor 硫酸氨基聚糖檢測試劑盒(Blyscan™ Glycosaminoglycan As

                      簡要描述:采用常規生化方法,通過標準曲線和比色,可定量分析總硫酸化多糖(氨基聚糖和蛋白聚糖)的含量,亦可測定O端和N端硫酸氨基多糖的比值。主要采用的是Blyscan染料(Biocolor公司的產品),它可以特異性的與氨基聚糖和蛋白聚糖上的硫酸多糖組分結合。

                      • 產品型號:B1000
                      • 廠商性質:代理商
                      • 更新時間:2024-10-29
                      • 訪  問  量:3291

                      產品介紹

                      Biocolor 硫酸氨基聚糖檢測試劑盒 
                      Biocolor Blyscan™ Glycosaminoglycan Assay

                       
                          采用常規生化方法,通過標準曲線和比色,可定量分析總硫酸化多糖(氨基聚糖和蛋白聚糖)的含量,亦可測定O端和N端硫酸氨基多糖的比值。主要采用的是Blyscan染料(Biocolor公司的產品),它可以特異性的與氨基聚糖和蛋白聚糖上的硫酸多糖組分結合。該方法不適于檢測已經降解的硫酸化雙糖片段。
                          該試劑盒可檢測廣泛的蛋白多糖,可檢測的氨基多糖包括硫酸軟骨素、硫酸角質素、硫酸皮膚素、硫酸肝素,不適合檢測包含海藻酸的樣品,因為它們含有糖醛酸。該試劑盒也不能對透明質酸定量,但透明質酸并不干擾對硫酸化氨基多糖的檢測。細胞培養基中20%的胎牛血清也不會干擾實驗。該方法檢測靈敏度為5ug,所需時間為1小時。
                       
                      檢測樣本:可來自軟骨組織、動脈組織、肺組織、皮膚組織、腫瘤組織、細胞核組織提取物、細胞培養液、羊水和尿液
                       
                      實驗操作 
                      1. 繪制標準曲線
                      2. 待測樣品的處理:
                      取50ul待測樣品進行實驗。如果反應物在656nm初的吸收峰大于1.5,則對樣品進行稀釋,待測樣樣品濃度落入標準曲線中段時,準確度較高。
                      3. 硫酸氨基聚糖—染料復合物的獲得
                      加1.0ml的Blyscan染料溶液到待測樣本中,充分混勻,待不溶物出現,離心,棄上清,則獲得紫色的硫酸氨基聚糖—染料復合物
                      4. 硫酸氨基聚糖—染料復合物中染料的釋放
                      加入1.0ml的染料釋放劑,充分混勻。溶解5min以上,使染料充分釋放
                      5. 測定
                      用分光光度計于波長656nm處測定,或使用colorimeter紅色濾光片測定
                       
                      產品訂購

                         Product Code: B1000     110 assays 
                         Product Code: B3000     440 assays

                       

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